Abstract
Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C-terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was isolated.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins*
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Catalysis
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Catalytic Domain
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Escherichia coli
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Gene Expression
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Glucosyltransferases / genetics*
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Mutagenesis
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Proteins / genetics*
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Streptococcus / enzymology*
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Streptococcus / genetics
Substances
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Bacterial Proteins
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Proteins
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GTF-I protein, Streptococcus
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Glucosyltransferases
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glucosyltransferase I