Selection analysis of single-stranded clones using solid-phase techniques

T Vener; A Holmberg; J Lundeberg; M Uhlén

doi:10.1016/s1050-3862(98)00035-7

Selection analysis of single-stranded clones using solid-phase techniques

Genet Anal. 1999 Apr;15(2):47-50. doi: 10.1016/s1050-3862(98)00035-7.

Authors

T Vener¹, A Holmberg, J Lundeberg, M Uhlén

Affiliation

¹ KTH, Royal Institute of Technology, Department of Biotechnology, Stockholm, Sweden. tvener@twt.com

PMID: 10191985
DOI: 10.1016/s1050-3862(98)00035-7

Abstract

An efficient selection analysis of single-stranded DNA fragments using subtractive hybridization on magnetic beads and fluorescence-based fragment analysis is described. This approach facilitates either direct cloning of captured fragments or removal of common clones from cDNA or shot-gun clone libraries.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA, Single-Stranded / analysis*
Humans
Mice
Nucleic Acid Hybridization / methods*

Substances

DNA, Single-Stranded