The Sox-13 gene: structure, promoter characterization, and chromosomal localization

Genomics. 1999 Apr 15;57(2):301-5. doi: 10.1006/geno.1999.5779.

Abstract

We have recently identified the HMG box transcription factor Sox-13 and described its expression during murine embryogenesis. Here we describe the structure of the murine Sox-13 gene. This gene spans approximately 12 kb and consists of 13 exons. The HMG domain is encoded by exons XI and XII, separated by an intron that is conserved among Sox-5, Sox-13, and Sox-17. A single major transcription initiation site was identified. Deletion analysis of the 3-kb promoter region revealed a 400-bp fragment driving transcription of a luciferase reporter in a Sox-13-expressing cell line. To determine the chromosomal localization of the human gene, a human SOX13 cDNA was isolated with 75% homology to the mouse Sox-13. FISH analysis mapped the human SOX13 gene to chromosome 1 band q32.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Autoantigens*
  • Base Sequence
  • Blotting, Northern
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1 / genetics
  • DNA / chemistry
  • DNA / genetics
  • Exons
  • Gene Expression Regulation
  • High Mobility Group Proteins / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Introns
  • Luciferases / genetics
  • Luciferases / metabolism
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA / genetics
  • RNA / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • SOXD Transcription Factors
  • Sequence Analysis, DNA
  • Tissue Distribution

Substances

  • Autoantigens
  • High Mobility Group Proteins
  • Recombinant Fusion Proteins
  • SOX13 protein, human
  • SOXD Transcription Factors
  • Sox13 protein, mouse
  • RNA
  • DNA
  • Luciferases

Associated data

  • GENBANK/AF057702
  • GENBANK/AF083105