Abstract
Bovine peripheral blood mononuclear cells from cattle immunized with a modified live virus bovine herpesvirus 1 (BHV1) vaccine and from BHV1-negative cattle were incubated in vitro with inactivated BHV1 for 6 days. Activation of T-lymphocyte subsets was measured by two-color flow cytometric analysis of T-cell phenotype and surface expression of CD25, the alpha-subunit of the high-affinity interleukin-2 receptor. Vaccinated animals, but not unvaccinated animals, had CD3+, CD4+, and gamma-delta T cells that significantly (p < 0.05) increased expression of CD25 when incubated with BHV1. CD8+ T cells from vaccinated animals did not consistently increase CD25 expression when incubated with inactivated BHV1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CD3 Complex / analysis
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / virology
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / metabolism
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CD8-Positive T-Lymphocytes / virology
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Cattle
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Cells, Cultured
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Epitopes, T-Lymphocyte / immunology*
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Flow Cytometry
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Herpesvirus 1, Bovine / immunology*
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Leukocytes, Mononuclear / immunology
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Lymphocyte Activation / immunology*
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Lymphocyte Count
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Male
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Receptors, Antigen, T-Cell, gamma-delta / immunology
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Receptors, Interleukin-2 / biosynthesis
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T-Lymphocyte Subsets / immunology*
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T-Lymphocyte Subsets / metabolism
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T-Lymphocyte Subsets / virology
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Vaccination / veterinary
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Vaccines, Attenuated / immunology
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Viral Vaccines / immunology*
Substances
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CD3 Complex
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Epitopes, T-Lymphocyte
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Receptors, Antigen, T-Cell, gamma-delta
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Receptors, Interleukin-2
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Vaccines, Attenuated
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Viral Vaccines