Antigen-specific in vitro activation of T-lymphocyte subsets of cattle immunized with a modified live bovine herpesvirus 1 vaccine

Viral Immunol. 1999;12(1):9-21. doi: 10.1089/vim.1999.12.9.

Abstract

Bovine peripheral blood mononuclear cells from cattle immunized with a modified live virus bovine herpesvirus 1 (BHV1) vaccine and from BHV1-negative cattle were incubated in vitro with inactivated BHV1 for 6 days. Activation of T-lymphocyte subsets was measured by two-color flow cytometric analysis of T-cell phenotype and surface expression of CD25, the alpha-subunit of the high-affinity interleukin-2 receptor. Vaccinated animals, but not unvaccinated animals, had CD3+, CD4+, and gamma-delta T cells that significantly (p < 0.05) increased expression of CD25 when incubated with BHV1. CD8+ T cells from vaccinated animals did not consistently increase CD25 expression when incubated with inactivated BHV1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD3 Complex / analysis
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • CD4-Positive T-Lymphocytes / virology
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / virology
  • Cattle
  • Cells, Cultured
  • Epitopes, T-Lymphocyte / immunology*
  • Flow Cytometry
  • Herpesvirus 1, Bovine / immunology*
  • Leukocytes, Mononuclear / immunology
  • Lymphocyte Activation / immunology*
  • Lymphocyte Count
  • Male
  • Receptors, Antigen, T-Cell, gamma-delta / immunology
  • Receptors, Interleukin-2 / biosynthesis
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocyte Subsets / metabolism
  • T-Lymphocyte Subsets / virology
  • Vaccination / veterinary
  • Vaccines, Attenuated / immunology
  • Viral Vaccines / immunology*

Substances

  • CD3 Complex
  • Epitopes, T-Lymphocyte
  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, Interleukin-2
  • Vaccines, Attenuated
  • Viral Vaccines