Terminal restriction fragment length polymorphism (T-RFLP): an emerging method for characterizing diversity among homologous populations of amplification products

T L Marsh

doi:10.1016/S1369-5274(99)80056-3

Terminal restriction fragment length polymorphism (T-RFLP): an emerging method for characterizing diversity among homologous populations of amplification products

Curr Opin Microbiol. 1999 Jun;2(3):323-7. doi: 10.1016/S1369-5274(99)80056-3.

Author

T L Marsh¹

Affiliation

¹ Center for Microbial Ecology, Department of Microbiology, Michigan State University, East Lansing, MI 48824, USA. MARSHT@pilot.msu.edu

PMID: 10383864
DOI: 10.1016/S1369-5274(99)80056-3

Abstract

Terminal restriction fragment length polymorphism is a recent molecular approach that can assess subtle genetic differences between strains as well as provide insight into the structure and function of microbial communities. The technique has both high sensitivity and throughput making it ideal for comparative analyses.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Ecosystem
Genetic Variation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length*
RNA, Ribosomal, 16S / classification
RNA, Ribosomal, 16S / genetics*

Substances

RNA, Ribosomal, 16S