Abstract
On infection, the herpes simplex virus (HSV) virion protein VP16 (Vmw65; alphaTIF) forms a transcriptional regulatory complex-the VP16-induced complex-with two cellular proteins, HCF and Oct-1, on VP16-responsive cis-regulatory elements in HSV immediate-early promoters called TAATGARAT. Comparison of different HSV VP16 sequences reveals a conserved core region that is sufficient for VP16-induced complex formation. The crystal structure of the VP16 core has been determined at 2.1 A resolution. The results reveal a novel, seat-like protein structure. Together with the activity of mutant VP16 proteins, the structure of free VP16 suggests that it contains (1) a disordered carboxy-terminal region that associates with HCF, Oct-1, and DNA in the VP16-induced complex, and (2) a structured region involved in virion assembly and possessing a novel DNA-binding surface that differentiates among TAATGARAT VP16-response elements.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Crystallization
-
Crystallography, X-Ray
-
DNA / metabolism
-
DNA-Binding Proteins / metabolism
-
Gene Expression Regulation, Viral
-
Herpes Simplex Virus Protein Vmw65 / chemistry*
-
Herpes Simplex Virus Protein Vmw65 / genetics
-
Herpes Simplex Virus Protein Vmw65 / metabolism
-
Herpesviridae / chemistry
-
Host Cell Factor C1
-
Macromolecular Substances
-
Models, Molecular
-
Molecular Sequence Data
-
Mutagenesis, Site-Directed
-
Octamer Transcription Factor-1
-
Protein Conformation*
-
Recombinant Fusion Proteins / genetics
-
Regulatory Sequences, Nucleic Acid
-
Sequence Alignment
-
Sequence Homology, Amino Acid
-
Simplexvirus / chemistry*
-
Simplexvirus / physiology
-
Transcription Factors / metabolism
-
Transcriptional Activation
Substances
-
DNA-Binding Proteins
-
Herpes Simplex Virus Protein Vmw65
-
Host Cell Factor C1
-
Macromolecular Substances
-
Octamer Transcription Factor-1
-
Recombinant Fusion Proteins
-
Transcription Factors
-
DNA