Crystallization and preliminary X-ray analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (tyrosine inhibitable) from Saccharomyces cerevisiae

Acta Crystallogr D Biol Crystallogr. 1999 Sep;55(Pt 9):1586-8. doi: 10.1107/s0907444999007830.

Abstract

3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase (E.C. 4.1.2.15) catalyses the first step in the biosynthesis of aromatic amino acids: the condensation of phophoenolpyruvate and erythrose 4-phosphate to 3-deoxy-D-arabino-heptulosonate-7-phosphate. Diffraction-quality crystals of the tyrosine-inhibitable form of the enzyme from Saccharomyces cerevisiae have been obtained by the hanging-drop vapour-diffusion method in the presence of polyethylene glycol. The crystals belong to the triclinic space group P1, with unit-cell parameters a = 81.5, b = 94.0, c = 104.6 A, alpha = 65.5, beta = 85.2, gamma = 75.0 degrees, and can be flash-cooled using glycerol as a cryoprotectant. A data set to 2.3 A has been collected at 120 K.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-Deoxy-7-Phosphoheptulonate Synthase / antagonists & inhibitors
  • 3-Deoxy-7-Phosphoheptulonate Synthase / chemistry*
  • 3-Deoxy-7-Phosphoheptulonate Synthase / isolation & purification
  • Crystallization
  • Enzyme Inhibitors / metabolism
  • Enzyme Inhibitors / pharmacology
  • Fungal Proteins / antagonists & inhibitors
  • Fungal Proteins / chemistry*
  • Fungal Proteins / isolation & purification
  • Saccharomyces cerevisiae / enzymology*
  • Tyrosine / metabolism
  • Tyrosine / pharmacology
  • X-Ray Diffraction

Substances

  • Enzyme Inhibitors
  • Fungal Proteins
  • Tyrosine
  • 3-Deoxy-7-Phosphoheptulonate Synthase