Molecular cloning and expression of human neurochondrin-1 and -2

Biochim Biophys Acta. 1999 Sep 3;1446(3):397-402. doi: 10.1016/s0167-4781(99)00120-7.

Abstract

Human neurochondrins have been cloned from a brain cDNA library. The human neurochondrin-1 and -2 predict leucine-rich (15.8 and 15.9%) proteins of 729 and 712 amino acid residues, with molecular weights of 78.9 and 77.2 kDa, respectively. The deduced amino acid sequence indicates 98% identity among human, mouse and rat species. Northern analysis indicates that about 4 kb human neurochondrin mRNAs are abundant in the fetal and the adult brain.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Brain / metabolism*
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification
  • Gene Expression
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics*
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • Sequence Alignment

Substances

  • DNA, Complementary
  • NCDN protein, human
  • Nerve Tissue Proteins
  • RNA, Messenger
  • neurochondrin-2

Associated data

  • GENBANK/AB018739
  • GENBANK/AB018740