Expression of mouse mammary tumor virus envelope protein does not prevent superinfection in vivo or in vitro

Virology. 1999 Oct 25;263(2):418-26. doi: 10.1006/viro.1999.9967.

Abstract

Inbred mice expressing endogenous mouse mammary tumor virus envelope proteins can be infected with exogenous virus, and the mammary tumors that develop in these mice usually have many proviruses integrated in their genomes, indicating that this virus is not subject to receptor interference. We show here that transgenic mice expressing an exogenous mouse mammary tumor virus (C3H) envelope protein can still be infected with this virus. Moreover, cultured mammary gland cells expressing the mouse mammary tumor virus (C3H) envelope protein can be superinfected with pseudotyped viruses bearing that same protein. Thus cellular expression of the mouse mammary tumor virus envelope protein does not block superinfection in vivo or in vitro.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line
  • DNA, Viral / analysis
  • DNA, Viral / genetics
  • Female
  • Mammary Glands, Animal / cytology
  • Mammary Glands, Animal / metabolism
  • Mammary Glands, Animal / pathology
  • Mammary Neoplasms, Experimental / metabolism
  • Mammary Neoplasms, Experimental / virology
  • Mammary Tumor Virus, Mouse / genetics
  • Mammary Tumor Virus, Mouse / metabolism*
  • Mice
  • Mice, Inbred C3H
  • Mice, Transgenic
  • Proviruses / genetics
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Retroviridae Infections / metabolism
  • Retroviridae Infections / virology*
  • Superinfection / metabolism
  • Superinfection / virology*
  • Transfection
  • Transgenes / genetics
  • Transgenes / physiology
  • Tumor Virus Infections / metabolism
  • Tumor Virus Infections / virology*
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / metabolism*

Substances

  • DNA, Viral
  • Recombinant Proteins
  • Viral Envelope Proteins