Abstract
The Aspergillus oryzae xynF1 gene coding for a xylanase, XynF1, was successfully overexpressed under the strong A. oryzae TEF1 gene promoter. The high-XynF1-producing transformant secreted about 180 mg/l of XynF1 in the glucose-containing medium. The overexpressed XynF1 was purified by only one chromatographic step. The purified XynF1 had a molecular mass of 35.0 kDa, a pH optimum of 5.0, and a temperature optimum of 60 degrees C.
MeSH terms
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Aspergillus oryzae / enzymology*
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Aspergillus oryzae / genetics
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Blotting, Southern
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Electrophoresis, Polyacrylamide Gel
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Gene Expression Regulation, Fungal*
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Hydrogen-Ion Concentration
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Models, Genetic
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Plasmids / genetics
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Promoter Regions, Genetic
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Temperature
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Xylan Endo-1,3-beta-Xylosidase
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Xylosidases / chemistry
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Xylosidases / genetics*
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Xylosidases / isolation & purification*
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Xylosidases / metabolism
Substances
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Xylosidases
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Xylan Endo-1,3-beta-Xylosidase