Abstract
Ephrin-A4 is a ligand for the erythropoietin-producing hepatocellular (Eph) receptor family of tyrosine kinases. We have identified a secreted form of ephrin-A4, denoted ephrin-A4 (s), which is encoded by an alternatively spliced mRNA and is produced by in vivo activated B cells in tonsils. Blood B cells secrete ephrin-A4 (s) upon stimulation via the B-cell antigen receptor. A subpopulation of tonsil cells in the crypts with a dendritic cell phenotype was shown to express EphA2, an Eph receptor tyrosine kinase that was found to be capable of binding an ephrin-A4 immunoglobulin chimeric protein. We conclude that ephrin-A4 (s) may play a role in the interaction between activated B lymphocytes and dendritic cells in human tonsils. (Blood. 2000;95:221-230)
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing*
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Amino Acid Sequence
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B-Lymphocytes / immunology
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B-Lymphocytes / physiology*
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Base Sequence
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Cell Line
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Cells, Cultured
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Cloning, Molecular
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Ephrin-A4
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Exons
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Genetic Variation*
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Glycosylphosphatidylinositols / metabolism
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Humans
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In Situ Hybridization
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Introns
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Jurkat Cells
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Lymphocyte Activation*
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Membrane Proteins / biosynthesis
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Membrane Proteins / chemistry
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Membrane Proteins / genetics*
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Molecular Sequence Data
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Palatine Tonsil / immunology
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription, Genetic*
Substances
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Ephrin-A4
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Glycosylphosphatidylinositols
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Membrane Proteins
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Recombinant Fusion Proteins
Associated data
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GENBANK/AJ006352
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GENBANK/AJ006353