Abstract
Proteins of the 14-3-3 family can associate with and/or modulate the activities of a variety of proteins, such as protooncogene and oncogene products, Cdc25 phosphatases and phosphatidylinositol 3-kinase, and thus are implicated in regulation of signaling pathways and the cell cycle. We report here that treatment of Jurkat T-cells with an inhibitor of protein tyrosine phosphatase, pervanadate, induces the association of 14-3-3tau with a translational control factor, FKBP12-rapamycin-associated protein (FRAP), with significant latter's autophosphorylation. Coimmunoprecipitation of various mutants of FRAP coexpressed with 14-3-3tau in COS-7 cells revealed that 14-3-3tau binds to the C-terminal side of FRAP at unknown site(s) different from the predicted binding motifs to date.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
14-3-3 Proteins
-
Amino Acid Substitution / genetics
-
Animals
-
Binding Sites
-
COS Cells
-
Carrier Proteins*
-
Humans
-
Immunophilins / chemistry*
-
Immunophilins / genetics
-
Immunophilins / metabolism*
-
Jurkat Cells
-
Lymphocyte Activation / drug effects
-
Mutation / genetics
-
Phosphorylation / drug effects
-
Phosphotransferases (Alcohol Group Acceptor)*
-
Precipitin Tests
-
Protein Binding / drug effects
-
Protein Isoforms / genetics
-
Protein Isoforms / metabolism
-
Protein Tyrosine Phosphatases / antagonists & inhibitors*
-
Protein Tyrosine Phosphatases / metabolism
-
Proteins / genetics
-
Proteins / metabolism*
-
Recombinant Fusion Proteins / chemistry
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
T-Lymphocytes / drug effects*
-
T-Lymphocytes / enzymology
-
T-Lymphocytes / metabolism
-
TOR Serine-Threonine Kinases
-
Tyrosine 3-Monooxygenase*
-
Vanadates / pharmacology*
Substances
-
14-3-3 Proteins
-
Carrier Proteins
-
Protein Isoforms
-
Proteins
-
Recombinant Fusion Proteins
-
pervanadate
-
Vanadates
-
Tyrosine 3-Monooxygenase
-
Phosphotransferases (Alcohol Group Acceptor)
-
MTOR protein, human
-
TOR Serine-Threonine Kinases
-
Protein Tyrosine Phosphatases
-
Immunophilins