Abstract
Expression of cyclooxygenase (COX)-2 protein in preneoplastic and neoplastic lung lesions induced by the administration of 2000 ppm of N-nitrosobis(2-hydroxypropyl)amine (BHP) in the drinking water to Wistar male rats, was examined immunohistochemically. The majority of alveolar/bronchiolar adenomas (ADs) and all adenocarcinomas (ADCs) examined, stained positive or strongly positive for COX-2. In contrast, only a minority of alveolar/bronchiolar hyperplasias demonstrated immunoreactivity and half of the squamous cell carcinomas examined, were only weakly positive. Western blotting analysis also revealed expression of COX-2 protein in the resected ADs and ADCs. These results clearly indicate up-regulated expression of COX-2 in lung neoplastic lesions, particularly ADs and ADCs, induced by BHP in rats.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma, Bronchiolo-Alveolar / chemically induced
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Adenocarcinoma, Bronchiolo-Alveolar / enzymology
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Animals
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Carcinogens / toxicity*
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Carcinoma, Non-Small-Cell Lung / chemically induced
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Carcinoma, Non-Small-Cell Lung / enzymology*
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Cyclooxygenase 1
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Cyclooxygenase 2
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Enzyme Induction / drug effects
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Growth Disorders / chemically induced
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Growth Disorders / enzymology
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Isoenzymes / biosynthesis*
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Isoenzymes / metabolism
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Lung Neoplasms / chemically induced
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Lung Neoplasms / enzymology*
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Male
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Membrane Proteins
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Mice
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Nitrosamines / toxicity*
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Precancerous Conditions / chemically induced
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Precancerous Conditions / enzymology
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Prostaglandin-Endoperoxide Synthases / biosynthesis*
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Prostaglandin-Endoperoxide Synthases / metabolism
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Rats
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Rats, Wistar
Substances
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Carcinogens
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Isoenzymes
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Membrane Proteins
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Nitrosamines
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diisopropanolnitrosamine
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Cyclooxygenase 1
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Cyclooxygenase 2
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Prostaglandin-Endoperoxide Synthases
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Ptgs1 protein, mouse
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Ptgs1 protein, rat