gC1q-R/p32, a C1q-binding protein, is a receptor for the InlB invasion protein of Listeria monocytogenes

EMBO J. 2000 Apr 3;19(7):1458-66. doi: 10.1093/emboj/19.7.1458.

Abstract

InlB is a Listeria monocytogenes protein that promotes entry of the bacterium into mammalian cells by stimulating tyrosine phosphorylation of the adaptor proteins Gab1, Cbl and Shc, and activation of phosphatidyl- inositol (PI) 3-kinase. Using affinity chromatography and enzyme-linked immunosorbent assay, we demonstrate a direct interaction between InlB and the mammalian protein gC1q-R, the receptor of the globular part of the complement component C1q. Soluble C1q or anti-gC1q-R antibodies impair InlB-mediated entry. Transient transfection of GPC16 cells, which are non-permissive to InlB-mediated entry, with a plasmid-expressing human gC1q-R promotes entry of InlB-coated beads. Furthermore, several experiments indicate that membrane recruitment and activation of PI 3-kinase involve an InlB-gC1q-R interaction and that gC1q-R associates with Gab1 upon stimulation of Vero cells with InlB. Thus, gC1q-R constitutes a cellular receptor involved in InlB-mediated activation of PI 3-kinase and tyrosine phosphorylation of the adaptor protein Gab1. After E-cadherin, the receptor for internalin, gC1q-R is the second identified mammalian receptor promoting entry of L. monocytogenes into mammalian cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Antibodies / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Binding, Competitive
  • Carrier Proteins
  • Cell Line
  • Chlorocebus aethiops
  • Complement C1q / metabolism*
  • DNA Primers / genetics
  • HeLa Cells
  • Humans
  • Hyaluronan Receptors*
  • Listeria monocytogenes / genetics
  • Listeria monocytogenes / metabolism*
  • Listeria monocytogenes / pathogenicity
  • Membrane Glycoproteins*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mitochondrial Proteins
  • Molecular Sequence Data
  • Phosphoproteins / metabolism
  • Phosphorylation
  • Receptors, Complement / antagonists & inhibitors
  • Receptors, Complement / genetics
  • Receptors, Complement / metabolism*
  • Transfection
  • Tyrosine / metabolism
  • Vero Cells

Substances

  • Adaptor Proteins, Signal Transducing
  • Antibodies
  • Bacterial Proteins
  • C1QBP protein, human
  • Carrier Proteins
  • DNA Primers
  • GAB1 protein, human
  • Hyaluronan Receptors
  • Membrane Glycoproteins
  • Membrane Proteins
  • Mitochondrial Proteins
  • Phosphoproteins
  • Receptors, Complement
  • complement 1q receptor
  • inlB protein, Listeria monocytogenes
  • Tyrosine
  • Complement C1q