Abstract
In the search for physiological substrates of MAPK-activated protein (MAPKAP) kinases, we identified the basic helix-loop-helix (bHLH) transcription factor E47 as an interaction partner of chromosome 3p kinase (3pK) and MAPKAP-K2 (MK2). The E2A protein E47 is known to be involved in the regulation of tissue-specific gene expression and cell differentiation. E47 is a phosphoprotein, and we identified 3pK and MK2 as E47 kinases in vitro. Furthermore, the expression of either kinase results in a repression of the transcriptional activity of E47 on an E-box containing promoter. In summary, the MAPK-activated protein kinases 3pK and MK2 were identified to form an assembly with the bHLH protein E47 suggesting that these kinases are regulators of E47 activity and E47-dependent gene expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cell Line
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Expression Regulation
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Genes, Reporter
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Humans
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Intracellular Signaling Peptides and Proteins
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MAP Kinase Kinase 2
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Mitogen-Activated Protein Kinase Kinases / metabolism*
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Molecular Sequence Data
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Phosphorylation
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Precipitin Tests
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Promoter Regions, Genetic
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Protein Binding
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Protein-Tyrosine Kinases / metabolism*
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Recombinant Proteins
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Repressor Proteins / metabolism*
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TCF Transcription Factors
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Transcription Factor 7-Like 1 Protein
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Transcription Factors*
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Transcriptional Activation / genetics
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Transfection
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Yeasts
Substances
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DNA-Binding Proteins
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Intracellular Signaling Peptides and Proteins
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Recombinant Proteins
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Repressor Proteins
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TCF Transcription Factors
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TCF7L1 protein, human
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Transcription Factor 7-Like 1 Protein
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Transcription Factors
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MAP-kinase-activated kinase 3
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MAP2K2 protein, human
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Protein-Tyrosine Kinases
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Protein Serine-Threonine Kinases
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MAP Kinase Kinase 2
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Mitogen-Activated Protein Kinase Kinases