Abstract
We have identified an interferon-like cytokine, limitin, on the basis of its ability to arrest the growth of or kill lympho-hematopoietic cells. Limitin strongly inhibited B lymphopoiesis in vitro and in vivo but had little influence on either myelopoiesis or erythropoiesis. Because limitin uses the interferon alpha/beta receptors and induces interferon regulatory factor-1, it may represent a previously unknown type I interferon prototype. However, preferential B-lineage growth inhibition and activation of Janus kinase 2 in a myelomonocytic leukemia line have not been described for previously known interferons.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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B-Lymphocytes / cytology*
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Base Sequence
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Bone Marrow / metabolism
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Cell Line, Transformed
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Cloning, Molecular
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Cytokines / analysis
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Cytokines / genetics
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Cytokines / physiology*
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DNA, Complementary
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DNA-Binding Proteins / metabolism
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Female
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Gene Expression
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Growth Inhibitors / genetics
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Growth Inhibitors / metabolism
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Hematopoietic Stem Cells / cytology*
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Humans
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Interferon Regulatory Factor-1
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Interferon Type I / chemistry
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Membrane Proteins
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Mice
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Mice, Inbred BALB C
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Mice, Knockout
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Molecular Sequence Data
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Phosphoproteins / metabolism
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Receptor, Interferon alpha-beta
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Receptors, Interferon / genetics
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Receptors, Interferon / metabolism
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Signal Transduction
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Spleen / metabolism
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Tumor Cells, Cultured
Substances
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Cytokines
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DNA, Complementary
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DNA-Binding Proteins
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Growth Inhibitors
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IRF1 protein, human
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Interferon Regulatory Factor-1
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Interferon Type I
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Irf1 protein, mouse
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Membrane Proteins
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Phosphoproteins
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Receptors, Interferon
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limitin
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Receptor, Interferon alpha-beta