Abstract
Diphenyl-1-pyrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP = O). DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor with tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several kinds of free radicals. It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Membrane / chemistry
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Cell Membrane / drug effects
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Cell Membrane / metabolism*
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Female
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism*
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Free Radicals / metabolism
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Hydrogen Peroxide / metabolism
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Lipid Peroxidation* / drug effects
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Lipid Peroxides / metabolism
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Liposomes / chemistry
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Liposomes / drug effects
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Liposomes / metabolism
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Mice
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Mice, Inbred ICR
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Microscopy, Fluorescence
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Neutrophils / cytology
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Neutrophils / drug effects
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Neutrophils / metabolism
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Organophosphorus Compounds / chemistry
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Organophosphorus Compounds / metabolism*
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Oxidants / metabolism
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Oxidation-Reduction / drug effects
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Phosphatidylcholines / metabolism
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Pyrenes / chemistry
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Pyrenes / metabolism*
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Solutions
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Spectrometry, Fluorescence
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Tetradecanoylphorbol Acetate / pharmacology
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tert-Butylhydroperoxide / metabolism
Substances
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Fluorescent Dyes
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Free Radicals
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Lipid Peroxides
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Liposomes
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Organophosphorus Compounds
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Oxidants
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Phosphatidylcholines
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Pyrenes
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Solutions
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diphenyl-1-pyrenylphosphine
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methyl linoleate hydroperoxide
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tert-Butylhydroperoxide
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Hydrogen Peroxide
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Tetradecanoylphorbol Acetate