Abstract
Using a conventional cloning technique, a novel full-length cDNA was isolated and sequenced from a human placental cDNA library. This cDNA consists of 2129 bp and has a predicted open reading frame encoding 366 amino acids. It possesses a Src homology 3 (SH3) motif, proline-rich region, serine-rich region and no catalytic domain, suggesting that it seems to be a signaling protein most similar to e3B1, an eps8 SH3 binding protein. PCR-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 17q21.3 near the marker D17S1795.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Amino Acid Sequence
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Base Sequence
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Carrier Proteins / genetics*
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Cell Line
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Chromosome Mapping
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Chromosomes, Human, Pair 17
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Cloning, Molecular
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Cytoskeletal Proteins
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DNA, Complementary / isolation & purification
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Gene Library
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Genes*
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Humans
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Molecular Sequence Data
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Polymerase Chain Reaction
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Protein Sorting Signals / genetics*
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Sequence Alignment
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Sequence Homology
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src Homology Domains
Substances
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ABI1 protein, human
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ABI3 protein, human
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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Cytoskeletal Proteins
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DNA, Complementary
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Protein Sorting Signals