Purification, crystallization and preliminary diffraction study of beta-galactosidase from Penicillium sp

Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1508-9. doi: 10.1107/s0907444900011756.

Abstract

Crystals of an extracellular beta-galactosidase from Penicillium sp. (MW = 120 +/- 5 kDa) have been obtained from a sodium phosphate buffer using PEG as precipitant. The crystals belong to the tetragonal space group P4(1)or P4(3), with unit-cell parameters a = b = 110.82, c = 161.28 A, and diffract to 1.85 A resolution at a synchrotron source.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallization
  • Crystallography, X-Ray
  • Glycosylation
  • Penicillium / enzymology*
  • Protein Conformation
  • beta-Galactosidase / chemistry
  • beta-Galactosidase / isolation & purification*
  • beta-Galactosidase / metabolism

Substances

  • beta-Galactosidase