Abstract
Using the yeast two hybrid system we have identified a novel protein termed somatostatin receptor interacting protein (SSTRIP) from human brain which interacts with the rat somatostatin receptor subtype 2. Interaction with the receptor C-terminus is mediated by a PSD-95/discs large/ZO-1 (PDZ) domain which exhibits high similarity to the PDZ domain of cortactin binding protein 1 (CortBP1). SSTRIP and CortBP1 define a novel family of multidomain proteins containing ankyrin repeats, SH3- and SH3 binding regions and a sterile alpha motif (SAM domain) in addition to the PDZ domain. Both SSTRIP and CortBP1 can be co-immunoprecipitated with the somatostatin receptor when co-expressed in HEK cells. Interestingly, co-localization of SSTR2 and CortBP1 at the plasma membrane is increased when SSTR2 is stimulated by agonists.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Ankyrins / chemistry
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Ankyrins / genetics
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Ankyrins / metabolism*
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Binding Sites / physiology
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Brain Chemistry / physiology
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Line
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Gene Expression / physiology
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Humans
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Kidney / cytology
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Phosphoproteins / chemistry
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Protein Structure, Tertiary
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Rats
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Receptors, Somatostatin / chemistry
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Receptors, Somatostatin / genetics
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Receptors, Somatostatin / metabolism*
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Two-Hybrid System Techniques
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Zonula Occludens-1 Protein
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src Homology Domains
Substances
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Ankyrins
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Carrier Proteins
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Membrane Proteins
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Nerve Tissue Proteins
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Phosphoproteins
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Receptors, Somatostatin
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Shank2 protein, rat
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TJP1 protein, human
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Tjp1 protein, rat
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Zonula Occludens-1 Protein
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somatostatin receptor 2