A serological survey has been carried out to detect evidence of hantavirus infection in rodents from two provinces of Thailand. This study aimed to examine virus antibody in 354 rodents trapped among 6 different villages of Nakhon Pathom Province (February-March, 1998) and in 326 rodents trapped among 14 villages of Nakhon Ratchasima Province (August-October, 1998). Seroprevalence among rodents from Nakhon Pathom Province (2.3%), was mostly find in Rattus norvegicus (3.8%) and Bandicota indica (2.6%). In Nakhon Ratchasima Province seroprevalence (4.0%) was mostly in Bandicota indica (19.1%) and Rattus exulans (3.5%).