Abstract
In this study, we report the complete cDNA sequence, genomic organization and expression pattern of the Caenorhabditis elegans ClC chloride channel gene, clh-6. Two different types of reporter gene fusions suggest that clh-6 expression is restricted to two gamma-aminobutyric acid-ergic RME neurons. These results are in striking contrast with the wide tissue distribution of messenger RNA for the related mammalian isoforms, CIC-6 and CIC-7. The restricted expression pattern of clh-6 provides a unique opportunity to study the biological function of a neuronal CIC chloride channel.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence / genetics
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Animals
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans Proteins*
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Carbocyanines / pharmacology
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Chloride Channels / genetics*
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Chloride Channels / metabolism
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Cloning, Molecular
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Fluorescent Dyes / pharmacology
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Gene Expression Regulation / physiology*
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Green Fluorescent Proteins
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Indicators and Reagents / analysis
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Luminescent Proteins / analysis
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Molecular Sequence Data
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Nervous System / cytology
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Nervous System / metabolism*
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Neurons / cytology
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Neurons / metabolism*
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Phylogeny
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Protein Isoforms / genetics
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Sequence Homology, Nucleic Acid
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gamma-Aminobutyric Acid / metabolism*
Substances
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3,3'-dihexadecylindocarbocyanine
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Caenorhabditis elegans Proteins
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Carbocyanines
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Chloride Channels
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Fluorescent Dyes
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Indicators and Reagents
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Luminescent Proteins
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Protein Isoforms
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clh-6 protein, C elegans
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Green Fluorescent Proteins
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gamma-Aminobutyric Acid