Rapid identification of Candida glabrata based on trehalose and sucrose assimilation using Rosco diagnostic tablets

J Lopez; F Dalle; P Mantelin; P Moiroux; A C Nierlich; A Pacot; B Cuisenier; O Vagner; A Bonnin

doi:10.1128/JCM.39.3.1172-1174.2001

Rapid identification of Candida glabrata based on trehalose and sucrose assimilation using Rosco diagnostic tablets

J Clin Microbiol. 2001 Mar;39(3):1172-4. doi: 10.1128/JCM.39.3.1172-1174.2001.

Authors

J Lopez¹, F Dalle, P Mantelin, P Moiroux, A C Nierlich, A Pacot, B Cuisenier, O Vagner, A Bonnin

Affiliation

¹ Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France.

Abstract

We developed a simple method for the identification of Candida glabrata on the basis of the ability of this species to rapidly assimilate trehalose but not sucrose. After incubation of yeasts with Rosco diagnostic tablets containing sucrose or trehalose, identification of C. glabrata was achieved in 4 h with 100% sensitivity and specificity.

Publication types

Evaluation Study

MeSH terms

Candida / classification*
Candida / metabolism*
Candidiasis / diagnosis
Candidiasis / microbiology
Humans
Mycological Typing Techniques
Sensitivity and Specificity
Sucrose / metabolism*
Tablets
Trehalose / metabolism*

Substances

Tablets
Sucrose
Trehalose