Simultaneous evaluation of dual gene transfer to adherent cells by gamma-ray imaging

Nucl Med Biol. 2001 Feb;28(2):135-44. doi: 10.1016/s0969-8051(00)00207-9.

Abstract

A gamma camera imaging method was developed to detect dual gene transfer to adherent cells growing as monolayers in cell culture plates. Human cancer cells were infected with replication-incompetent adenoviral vectors encoding the human type 2 somatostatin receptor (Ad-hSSTr2) and/or herpes virus thymidine kinase (Ad-TK). The hSSTr2 and TK reporter proteins were detected by imaging internally bound (99m)Tc-P2045 peptide (Diatide, Inc.) and radioiodinated 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-5-iodouracil (FIAU), respectively. Following gene transfer, expression of hSSTr2 and TK were accurately imaged in vitro.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae
  • Arabinofuranosyluracil / analogs & derivatives*
  • Arabinofuranosyluracil / pharmacokinetics
  • Carcinoma, Non-Small-Cell Lung
  • Cell Adhesion
  • Cell Line
  • Gamma Cameras
  • Gamma Rays
  • Genetic Vectors
  • Herpesviridae / genetics
  • Humans
  • Iodine Radioisotopes / pharmacokinetics
  • Kidney
  • Lung Neoplasms
  • Radiopharmaceuticals
  • Receptors, Somatostatin / genetics*
  • Technetium / pharmacokinetics
  • Thymidine Kinase / genetics*
  • Transfection / methods*
  • Tumor Cells, Cultured

Substances

  • Iodine Radioisotopes
  • Radiopharmaceuticals
  • Receptors, Somatostatin
  • Arabinofuranosyluracil
  • fialuridine
  • Technetium
  • somatostatin receptor 2
  • Thymidine Kinase