Abstract
Denture biofilms represent a protective reservoir for oral microbes. The study of the biology of Candida in these biofilms requires a reliable model. A reproducible model of C. albicans denture biofilm was developed and used to determine the susceptibility of two clinically relevant C. albicans isolates against 4 antifungals. C. albicans, growing as a biofilm, exhibited resistance to amphotericin B, nystatin, chlorhexidine, and fluconazole, with 50% reduction in metabolic activity (50% RMA) at concentrations of 8, 16, 128, and > 64 microg/mL, respectively. In contrast, planktonically cultured C. albicans were susceptible (50% RMA for the same antifungals was obtained at 0.25, 1.0, 4.0, and 0.5 microg/mL, respectively). In conclusion, results obtained by means of our biofilm model show that biofilm-associated C. albicans cells, compared with cells grown in planktonic form, are resistant to antifungals used to treat denture stomatitis.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acrylic Resins*
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Amphotericin B / administration & dosage
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Amphotericin B / pharmacology
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Anti-Infective Agents, Local / administration & dosage
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Anti-Infective Agents, Local / pharmacology
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Antifungal Agents / administration & dosage
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Antifungal Agents / pharmacology*
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Biofilms / drug effects*
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Candida albicans / classification
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Candida albicans / drug effects*
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Candidiasis, Oral / drug therapy
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Candidiasis, Oral / microbiology
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Chlorhexidine / administration & dosage
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Chlorhexidine / pharmacology
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Colony Count, Microbial
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Denture Bases / microbiology*
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Dose-Response Relationship, Drug
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Drug Resistance, Microbial
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Fluconazole / administration & dosage
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Fluconazole / pharmacology
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Galactose / pharmacology
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Glucose / pharmacology
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Humans
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Indicators and Reagents
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Nystatin / administration & dosage
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Nystatin / pharmacology
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Polymethyl Methacrylate
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Saliva / microbiology
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Statistics as Topic
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Stomatitis, Denture / drug therapy
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Stomatitis, Denture / microbiology
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Sucrose / pharmacology
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Tetrazolium Salts
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Time Factors
Substances
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Acrylic Resins
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Anti-Infective Agents, Local
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Antifungal Agents
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Indicators and Reagents
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Tetrazolium Salts
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2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide
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Nystatin
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Sucrose
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Amphotericin B
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Fluconazole
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Polymethyl Methacrylate
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Glucose
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Chlorhexidine
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Galactose