Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660

X Y Zhang; A L Dai; K Kuroiwa; R Kodaira; M Nogawa; M Shimosaka; M Okazaki

doi:10.1271/bbb.65.977

Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660

Biosci Biotechnol Biochem. 2001 Apr;65(4):977-81. doi: 10.1271/bbb.65.977.

Authors

X Y Zhang¹, A L Dai, K Kuroiwa, R Kodaira, M Nogawa, M Shimosaka, M Okazaki

Affiliation

¹ Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.

PMID: 11388486
DOI: 10.1271/bbb.65.977

Abstract

A genomic copy of the gene coding for chitosanase (csnA) was isolated from Aspergillus oryzae IAM 2660. A. oryzae csnA contains an open reading frame that encodes a polypeptide of 245 amino acids with a calculated molecular mass of 26,500 Da. The deduced amino acid sequence of A. oryzae csnA indicates extensive similarities to those of other fungal chitosanases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Aspergillus fumigatus / enzymology
Aspergillus fumigatus / genetics
Aspergillus oryzae / enzymology*
Aspergillus oryzae / genetics*
Cloning, Molecular
Fusarium / enzymology
Fusarium / genetics
Gene Library
Glycoside Hydrolases / genetics*
Molecular Sequence Data
Reverse Transcriptase Polymerase Chain Reaction
Species Specificity

Substances

Glycoside Hydrolases
chitosanase

Associated data

GENBANK/AF105078