Escherichia coli FtsZ polymers contain mostly GTP and have a high nucleotide turnover

Mol Microbiol. 2001 Jul;41(1):83-91. doi: 10.1046/j.1365-2958.2001.02498.x.

Abstract

The cell division protein FtsZ is a GTPase structurally related to tubulin and, like tubulin, it assembles in vitro into filaments, sheets and other structures. To study the roles that GTP binding and hydrolysis play in the dynamics of FtsZ polymerization, the nucleotide contents of FtsZ were measured under different polymerizing conditions using a nitrocellulose filter-binding assay, whereas polymerization of the protein was followed in parallel by light scattering. Unpolymerized FtsZ bound 1 mol of GTP mol(-1) protein monomer. At pH 7.5 and in the presence of Mg(2+) and K(+), there was a strong GTPase activity; most of the bound nucleotide was GTP during the first few minutes but, later, the amount of GTP decreased in parallel with depolymerization, whereas the total nucleotide contents remained invariant. These results show that the long FtsZ polymers formed in solution contain mostly GTP. Incorporation of nucleotides into the protein was very fast either when the label was introduced at the onset of the reaction or subsequently during polymerization. Molecular modelling of an FtsZ dimer showed the presence of a cleft between the two subunits maintaining the nucleotide binding site open to the medium. These results show that the FtsZ polymers are highly dynamic structures that quickly exchange the bound nucleotide, and this exchange can occur in all the subunits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Bacterial Proteins / ultrastructure
  • Biopolymers / chemistry
  • Biopolymers / metabolism*
  • Cytoskeletal Proteins*
  • Dimerization
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • GTP Phosphohydrolases / metabolism
  • Guanosine Triphosphate / metabolism*
  • Magnesium / metabolism
  • Microscopy, Electron
  • Models, Molecular
  • Nucleotides / metabolism*
  • Potassium / metabolism

Substances

  • Bacterial Proteins
  • Biopolymers
  • Cytoskeletal Proteins
  • FtsZ protein, Bacteria
  • Nucleotides
  • Guanosine Triphosphate
  • GTP Phosphohydrolases
  • Magnesium
  • Potassium