Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al. 1998 emend

Int J Syst Evol Microbiol. 2001 Sep;51(Pt 5):1773-1782. doi: 10.1099/00207713-51-5-1773.

Abstract

Thirty-one heavy-metal-resistant bacteria isolated from industrial biotopes were subjected to polyphasic characterization, including 16S rDNA sequence analysis, DNA-DNA hybridizations, biochemical tests, whole-cell protein and fatty-acid analyses. All strains were shown to belong to the Ralstonia branch of the beta-Proteobacteria. Whole-cell protein profiles and DNA-DNA hybridizations revealed two clearly distinct groups, showing low similarity to known Ralstonia species. These two groups, of 8 and 17 isolates, were assigned to two new species, for which the names Ralstonia campinensis sp. nov. and Ralstonia metallidurans sp. nov. are proposed. The type strains are WS2T (= LMG 19282T = CCUG 44526T) and CH34T (= LMG 1195T = DSM 2839T), respectively. Six isolates were allocated to Ralstonia basilensis, which presently contains only the type strain; an emendation of the latter species description is therefore proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry
  • Base Composition
  • Betaproteobacteria / classification*
  • Betaproteobacteria / drug effects*
  • Betaproteobacteria / metabolism
  • DNA, Ribosomal / analysis
  • Drug Resistance, Bacterial*
  • Electrophoresis, Polyacrylamide Gel
  • Fatty Acids / analysis
  • Industrial Microbiology*
  • Metals, Heavy / pharmacology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phenotype
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins
  • DNA, Ribosomal
  • Fatty Acids
  • Metals, Heavy
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AF312020
  • GENBANK/AF312021
  • GENBANK/AF312022