Abstract
Site-specific recombinases have become powerful tools for the targeted integration of transgenes into defined chromosomal loci. They have been successfully used both to achieve predictable gene expression in cell culture and for the systematic creation of transgenic animals. A recent improvement of this method, the recombinase-mediated cassette exchange procedure (RMCE), permits expression in the absence of any co-expressed selection marker gene.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Animals
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DNA Nucleotidyltransferases / genetics*
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DNA Nucleotidyltransferases / physiology
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Gene Targeting / methods*
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Integrases / genetics
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Integrases / physiology
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Kinetics
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Mutagenesis, Insertional / methods*
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Mutagenesis, Site-Directed
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Promoter Regions, Genetic
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Recombination, Genetic
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Thermodynamics
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Transgenes*
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Viral Proteins / genetics
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Viral Proteins / physiology
Substances
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Viral Proteins
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Cre recombinase
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DNA Nucleotidyltransferases
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FLP recombinase
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Integrases