Assay of Cdc42, Rac, and Rho GTPase activation by affinity methods

Methods Enzymol. 2002:345:349-59. doi: 10.1016/s0076-6879(02)45028-8.

Authors

Valerie Benard¹, Gary M Bokoch

Affiliation

¹ UPRESA 5018-CNRS, 31054 Toulouse, France.

PMID: 11665618
DOI: 10.1016/s0076-6879(02)45028-8

No abstract available

MeSH terms

Animals
Binding Sites
Chemical Precipitation
GTP Phosphohydrolases / analysis
GTP Phosphohydrolases / metabolism
In Vitro Techniques
Kinetics
Protein Kinases / chemistry
Protein Kinases / metabolism
Protein Structure, Tertiary
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / metabolism
cdc42 GTP-Binding Protein / analysis*
cdc42 GTP-Binding Protein / metabolism*
rac GTP-Binding Proteins / analysis*
rac GTP-Binding Proteins / metabolism*
rho GTP-Binding Proteins / analysis*
rho GTP-Binding Proteins / metabolism*

Substances

Recombinant Fusion Proteins
Protein Kinases
protease activated kinase I
GTP Phosphohydrolases
cdc42 GTP-Binding Protein
rac GTP-Binding Proteins
rho GTP-Binding Proteins