Separation and quantitation of bile acids using an isocratic solvent system for high performance liquid chromatography coupled to an evaporative light scattering detector

E C Torchia; E D Labonté; L B Agellon

doi:10.1006/abio.2001.5379

Separation and quantitation of bile acids using an isocratic solvent system for high performance liquid chromatography coupled to an evaporative light scattering detector

Anal Biochem. 2001 Nov 15;298(2):293-8. doi: 10.1006/abio.2001.5379.

Authors

E C Torchia¹, E D Labonté, L B Agellon

Affiliation

¹ Department of Biochemistry and Canadian Institutes of Health Research Group in Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta, T6G 2S2, Canada.

PMID: 11700985
DOI: 10.1006/abio.2001.5379

Abstract

We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bile / chemistry*
Bile Acids and Salts / analysis*
Bile Acids and Salts / isolation & purification*
Chromatography, High Pressure Liquid / methods*
Gallbladder / chemistry
Humans
Light
Scattering, Radiation
Solvents

Substances

Bile Acids and Salts
Solvents