Abstract
The Clostridium josui aga27A gene encodes the cellulosomal alpha-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various alpha-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A alpha-galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan as a substrate to small saccharides such as melibiose and raffinose.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cellulase
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Clostridium / enzymology*
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Clostridium / genetics
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DNA, Bacterial
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Galactose / analogs & derivatives
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Humans
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Mannans / metabolism
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Mice
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Molecular Sequence Data
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Mutagenesis
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Organelles / enzymology
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Sequence Analysis, DNA
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Sequence Analysis, Protein
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Sequence Homology, Amino Acid
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Substrate Specificity
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alpha-Galactosidase / genetics
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alpha-Galactosidase / isolation & purification
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alpha-Galactosidase / metabolism*
Substances
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DNA, Bacterial
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Mannans
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galactomannan
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alpha-Galactosidase
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Cellulase
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Galactose