Abstract
A novel gene was identified 52 kb upstream of the gene encoding the protease inhibitor elafin (PI3) on human chromosome 20q12-13.1. The transcript of the new gene, denoted huWAP2, was characterized by rapid amplification of cDNA ends and DNA sequencing. The size is 774 bp and it gives rise to a polypeptide of 111 amino acid residues that is homologous to elafin and similar WAP-type protease inhibitors. By RT-PCR it was shown that the gene is highly expressed in prostate, skin, lung, and esophagus.
(c)2002 Elsevier Science.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Chromosomes, Human, Pair 20
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DNA, Complementary / metabolism
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Elafin
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Exons
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Humans
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Male
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Molecular Sequence Data
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Prostate / metabolism*
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Protease Inhibitors / chemistry*
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Protein Biosynthesis*
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Protein Structure, Tertiary
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Proteinase Inhibitory Proteins, Secretory
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Proteins / chemistry
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Proteins / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Tissue Distribution
Substances
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DNA, Complementary
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Elafin
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PI3 protein, human
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Protease Inhibitors
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Proteinase Inhibitory Proteins, Secretory
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Proteins
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WFDC12 protein, human