Abstract
The stem-loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells. SLBP binds histone pre-mRNAs and facilitates 3'-end processing by promoting stable association of U7 snRNP with the pre-mRNA. To identify other factors involved in histone pre-mRNA processing, we used a modified yeast two-hybrid assay in which SLBP and its RNA target were coexpressed as bait. A novel zinc finger protein, hZFP100, which interacts with the SLBP/RNA complex but not with free SLBP, was cloned. The interaction requires regions of SLBP that are important for histone pre-mRNA processing. Antibodies to hZFP100 precipitate U7 snRNA, and expression of hZFP100 in Xenopus oocytes stimulates processing of histone pre-mRNA, showing that hZFP100 is a component of the processing machinery.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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HeLa Cells
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Histones / genetics*
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Histones / metabolism
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Humans
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Molecular Sequence Data
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Peptide Mapping
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RNA Precursors / metabolism*
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RNA Processing, Post-Transcriptional*
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RNA-Binding Proteins / metabolism*
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Ribonucleoprotein, U7 Small Nuclear / metabolism*
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Xenopus
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Xenopus Proteins*
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Zinc Fingers
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mRNA Cleavage and Polyadenylation Factors*
Substances
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DNA-Binding Proteins
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Histones
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Nuclear Proteins
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RNA Precursors
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RNA-Binding Proteins
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Ribonucleoprotein, U7 Small Nuclear
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SLBP protein, human
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SLBP1 protein, Xenopus
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Xenopus Proteins
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ZNF473 protein, human
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mRNA Cleavage and Polyadenylation Factors