An antibody that prevents the hemagglutinin low pH fusogenic transition

C Barbey-Martin; B Gigant; T Bizebard; L J Calder; S A Wharton; J J Skehel; M Knossow

doi:10.1006/viro.2001.1320

An antibody that prevents the hemagglutinin low pH fusogenic transition

Virology. 2002 Mar 1;294(1):70-4. doi: 10.1006/viro.2001.1320.

Authors

C Barbey-Martin¹, B Gigant, T Bizebard, L J Calder, S A Wharton, J J Skehel, M Knossow

Affiliation

¹ Laboratoire d'Enzymologie et Biochimie Structurales, UPR 9063 CNRS, Bât. 34, CNRS, 91198 Gif-sur-Yvette Cedex, France.

PMID: 11886266
DOI: 10.1006/viro.2001.1320

Abstract

We have determined the structure of a complex of influenza hemagglutinin (HA) with an antibody that binds simultaneously to the membrane-distal domains of two HA monomers, effectively cross-linking them. The antibody prevents the low pH structural transition of HA that is required for its membrane fusion activity, providing evidence that a rearrangement of HA membrane-distal domains is an essential component of the transition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral / immunology
Antibody Affinity
Hemagglutinin Glycoproteins, Influenza Virus / chemistry
Hemagglutinin Glycoproteins, Influenza Virus / immunology*
Hemagglutinin Glycoproteins, Influenza Virus / physiology
Hydrogen-Ion Concentration
Immunoglobulin Fab Fragments / immunology*
Membrane Fusion*
Models, Molecular
Orthomyxoviridae / physiology*
Protein Conformation

Substances

Antibodies, Viral
Hemagglutinin Glycoproteins, Influenza Virus
Immunoglobulin Fab Fragments

Associated data

PDB/1KEN