Abstract
We evaluated the association of nasopharyngeal carcinoma (NPC) and Epstein-Barr virus (EBV) in Spanish patients, and studied the expression of EBV products (latent membrane protein-1 [LMP-1] and ZEBRA proteins) by NPC cells and its possible prognostic value. In situ hybridization (ISH) for EBV-encoded nonpolyadenylated RNAs (EBERs) and immunohistochemical expression of LMP-1 and ZEBRA proteins by immunohistochemistry were examined in formalin-fixed, paraffin-embedded NPC specimens from 30 patients, and a survival analysis was done by the Kaplan-Meier method. We detected EBERs by ISH in 96.67% of the NPC cases, and detected expression of LMP-I in 43.33% of the NPC cases and expression of ZEBRA protein in 6.67% of the NPC cases. We conclude that ISH for expression of EBERs is an adequate method for detection of EBV in NPC. LMP-1 is not frequently expressed in NPC cells (43.33%). Most NPC cells carry a latent EBV infection. LMP-1 expression might have worsened the prognosis of NPC in our series.
MeSH terms
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Adolescent
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Adult
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Age of Onset
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Aged
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Aged, 80 and over
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / genetics
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Epstein-Barr Virus Infections / complications*
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Epstein-Barr Virus Infections / diagnosis
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Female
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Follow-Up Studies
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Gene Expression Regulation, Neoplastic / genetics*
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Herpesvirus 4, Human / genetics
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Humans
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Immunohistochemistry
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In Situ Hybridization
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Male
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Middle Aged
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Nasopharyngeal Neoplasms / epidemiology
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Nasopharyngeal Neoplasms / genetics*
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Nasopharyngeal Neoplasms / pathology
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Nasopharyngeal Neoplasms / virology*
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Neoplasm Staging
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Prognosis
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Proportional Hazards Models
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RNA, Viral / analysis
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RNA, Viral / genetics
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RNA-Binding Proteins / analysis
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RNA-Binding Proteins / genetics
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Ribosomal Proteins*
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Spain / epidemiology
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Survival Analysis
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Trans-Activators / analysis
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Trans-Activators / genetics
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Viral Matrix Proteins / analysis
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Viral Matrix Proteins / genetics
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Viral Proteins*
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White People / genetics*
Substances
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BZLF1 protein, Herpesvirus 4, Human
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DNA-Binding Proteins
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EBV-associated membrane antigen, Epstein-Barr virus
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RNA, Viral
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RNA-Binding Proteins
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Ribosomal Proteins
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Trans-Activators
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Viral Matrix Proteins
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Viral Proteins
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RPL22 protein, human