We report an endogenous signal that has a widespread cortical distribution and is time-locked to the termination of a sustained state of task-readiness. In three event-related functional magnetic resonance imaging (fMRI) experiments, subjects saw an arrow cue that predicted either the direction of motion or the location of a subsequent test stimulus. A reactivation of the BOLD (blood oxygenation level-dependent) signal occurred at the termination of the state of readiness in occipital regions that were transiently activated by the cue and in frontal-parietal regions that maintained an attentional set over the trial. Moreover, a delayed activation occurred in prefrontal and temporo-parietal regions that did not initially respond to the cue and that have been implicated in re-orienting attention to novel sensory events. These latter regions may have generated control signals that ended the state of readiness in regions active during the cue period. These results indicate that terminating a state of readiness produces a widely distributed cortical signal and suggest that areas involved in a preparatory state may be maintained as a network which can be modulated as a whole.