Abstract
A novel human kinase gene, human MRK (hMRK), was cloned by using degenerate RT-PCR. The hMRK encoded a putative 632 amino acids protein and was highly homologous to rat MRK (rMRK) in the entire coding region. The hMRK was located at chromosome 6p12.3 by RH-PCR analysis. The hMRK was generally expressed a single approximately 6.3 kb transcript at a low level in a variety of tissues except at a high level in testis. The full-length hMRK protein was fused to C-terminal of GFP and expressed in Hela cells. The fluorescence microscopy results identified its nuclear localization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Chromosome Mapping
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Chromosomes, Human
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Chromosomes, Human, Pair 6 / genetics
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Cloning, Molecular
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Gene Expression*
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Humans
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Molecular Sequence Data
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Protein Engineering / methods*
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Protein Serine-Threonine Kinases / chemical synthesis*
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Protein Serine-Threonine Kinases / genetics*
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Rats
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Recombinant Fusion Proteins
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Analysis, Protein / methods*
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Sequence Homology
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Tissue Distribution
Substances
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Recombinant Fusion Proteins
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CILK1 protein, human
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Cilk1 protein, rat
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Protein Serine-Threonine Kinases