Abstract
The 3' Ig heavy chain locus (Igh) regulatory region is the most downstream known element of the murine Igh gene cluster. We report here that the nearest non-Igh genes-Crip, Crp2, and Mta1-are located approximately 70 kb further downstream and are beyond the end of the domain of Igh transcriptional regulation. We have localized an origin of replication in MEL cells to a 3-kb segment located between the 3' Igh regulatory region and Crip. Sequences downstream of this origin are replicated by forks that move in both directions. Sequences upstream of this origin (Igh-C, -D, and -J) are replicated in a single direction through a 500-kb segment in which no active bidirectional origins can be detected. We propose that this origin may lie at or near the end of the Igh regulation domain.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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CCAAT-Enhancer-Binding Protein-beta / genetics
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Carrier Proteins / genetics
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Cell Line
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Chromosomes, Artificial, Bacterial / genetics
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DNA Replication / genetics
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Expressed Sequence Tags
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Gene Expression Regulation
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Histone Deacetylases*
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Immunoglobulin A / genetics
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Immunoglobulin Heavy Chains / genetics*
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LIM Domain Proteins
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Mice
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Molecular Sequence Data
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Multigene Family
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Proteins / genetics
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Replication Origin
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Replicon / genetics*
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Repressor Proteins*
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Trans-Activators
Substances
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CCAAT-Enhancer-Binding Protein-beta
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CRIP1 protein, human
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Carrier Proteins
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Crip1 protein, mouse
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Immunoglobulin A
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Immunoglobulin Heavy Chains
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LIM Domain Proteins
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MTA1 protein, human
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Proteins
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Repressor Proteins
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Trans-Activators
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Histone Deacetylases
Associated data
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GENBANK/AF450245
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GENBANK/AF463504