Molecular cloning and sequencing of the human heme-regulated eukaryotic initiation factor 2 alpha (eIF-2 alpha) kinase from bone marrow culture

Takeshi Omasa; Yi-Guang Chen; Athanasios Mantalaris; Ying-Chuech Tsai; J H David Wu

doi:10.1080/10425170290023428

Molecular cloning and sequencing of the human heme-regulated eukaryotic initiation factor 2 alpha (eIF-2 alpha) kinase from bone marrow culture

DNA Seq. 2002 Jun;13(3):133-7. doi: 10.1080/10425170290023428.

Authors

Takeshi Omasa¹, Yi-Guang Chen, Athanasios Mantalaris, Ying-Chuech Tsai, J H David Wu

Affiliation

¹ Department of Chemical Engineering, University of Rochester, 206 Gavett Hall, Rochester, New York 14627-0166, USA.

PMID: 12391722
DOI: 10.1080/10425170290023428

Abstract

The cDNA encoding human heme-regulated eukaryotic initiation factor-2 alpha (eIF-2 alpha) kinase was cloned from a human bone marrow culture. Its deduced amino acid sequence comprised of 629 amino acids with a calculated molecular weight of 71,031 Da. RT-PCR analysis revealed that the gene was also expressed in heart, kidney, spleen, muscle, and stomach.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Bone Marrow Cells
Cloning, Molecular
Conserved Sequence
Humans
Mice
Molecular Sequence Data
Organ Specificity
Rabbits
Rats
Sequence Alignment
eIF-2 Kinase / genetics*

Substances

eIF-2 Kinase