rDNA (18S-28S and 5S) colocalization and linkage between ribosomal genes and (TTAGGG)(n) telomeric sequence in the earthworm, Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae), revealed by single- and double-color FISH

R Vitturi; M S Colomba; A M Pirrone; M Mandrioli

doi:10.1093/jhered/93.4.279

rDNA (18S-28S and 5S) colocalization and linkage between ribosomal genes and (TTAGGG)(n) telomeric sequence in the earthworm, Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae), revealed by single- and double-color FISH

J Hered. 2002 Jul-Aug;93(4):279-82. doi: 10.1093/jhered/93.4.279.

Authors

R Vitturi¹, M S Colomba, A M Pirrone, M Mandrioli

Affiliation

¹ Dipartimento di Biologia Animale, Università di Palermo, Palermo, Italy. zuvitcol@unipa.it

PMID: 12407215
DOI: 10.1093/jhered/93.4.279

Abstract

Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Annelida: Oligochaeta) were examined using fluorescent in situ hybridization (FISH) with three repetitive DNA probes-5S rDNA, 18S-26S rDNA, and (TTAGGG)(n). Single-color FISH consistently mapped one chromosome pair per spread using either 5S rDNA or 18S-26S rDNA as probes. Simultaneous (18S-26S)-5S and (18S-26S)-(TTAGGG)(n) FISH demonstrated that repeated units of the two ribosomal families were overlapped and closely associated with telomeric sequences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA, Ribosomal*
Genetic Linkage*
In Situ Hybridization, Fluorescence
Oligochaeta / genetics*
Telomere*

Substances

DNA, Ribosomal