Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop

Nat Genet. 2003 Jan;33(1):23-4. doi: 10.1038/ng1064. Epub 2002 Dec 23.

Abstract

Human mitochondrial transcription factor B1 (h-mtTFB1) has an unprecedented relationship to RNA methyltransferases. Here, we show that this protein methylates a conserved stem-loop in bacterial 16S rRNA and that the homologous sequence in the human mitochondrial 12S molecule is similarly modified. Thus, h-mtTFB1 appears to be dual-function protein, acting both as a transcription factor and an rRNA-modification enzyme.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminoglycosides*
  • Anti-Bacterial Agents / pharmacology
  • Base Sequence
  • Conserved Sequence
  • Escherichia coli / drug effects
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Gene Deletion
  • Humans
  • Methylation
  • Methyltransferases / genetics
  • Mitochondria / genetics*
  • Nucleic Acid Conformation*
  • RNA, Bacterial / chemistry
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism
  • RNA, Ribosomal / chemistry*
  • RNA, Ribosomal / genetics
  • RNA, Ribosomal / metabolism*
  • RNA, Ribosomal, 16S / chemistry
  • RNA, Ribosomal, 16S / genetics
  • RNA, Ribosomal, 16S / metabolism
  • Transcription Factors / metabolism*
  • Upstream Stimulatory Factors

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • B1 factor, Xenopus
  • RNA, Bacterial
  • RNA, Ribosomal
  • RNA, Ribosomal, 16S
  • RNA, ribosomal, 12S
  • Transcription Factors
  • Upstream Stimulatory Factors
  • Methyltransferases
  • 16S rRNA (adenine(1518)-N(6)-adenine(1519)-N(6))-dimethyltransferase
  • kasugamycin