Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP

C Fanello; F Santolamazza; A della Torre

doi:10.1046/j.1365-2915.2002.00393.x

Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP

Med Vet Entomol. 2002 Dec;16(4):461-4. doi: 10.1046/j.1365-2915.2002.00393.x.

Authors

C Fanello¹, F Santolamazza, A della Torre

Affiliation

¹ London School of Hygiene and Tropical Medicine, London, UK.

PMID: 12510902
DOI: 10.1046/j.1365-2915.2002.00393.x

Abstract

For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Africa
Aging
Animals
Anopheles / classification*
Anopheles / genetics*
Base Sequence
Genes, Insect*
Larva / classification
Larva / genetics
Polymerase Chain Reaction*
Polymorphism, Restriction Fragment Length*
Species Specificity