Comparison of serological assays for detection of Chlamydia trachomatis antibodies in different groups of obstetrical and gynecological patients

C J Bax; J A E M Mutsaers; C L Jansen; J B Trimbos; P J Dörr; P M Oostvogel

doi:10.1128/cdli.10.1.174-176.2003

Comparison of serological assays for detection of Chlamydia trachomatis antibodies in different groups of obstetrical and gynecological patients

Clin Diagn Lab Immunol. 2003 Jan;10(1):174-6. doi: 10.1128/cdli.10.1.174-176.2003.

Authors

C J Bax¹, J A E M Mutsaers, C L Jansen, J B Trimbos, P J Dörr, P M Oostvogel

Affiliation

¹ Department of Obstetrics and Gynecology, MCH Westeinde Hospital, The Hague, The Netherlands. c.bax@mchaaglanden.nl

Abstract

New serological enzyme immunoassays (EIAs) were compared with microimmunofluorescence (MIF) as a "gold standard" to detect Chlamydia trachomatis antibodies in different groups of obstetrical, gynecological, and subfertile patients. There were no significant differences in seroprevalence rates, except for the group of C. trachomatis-positive patients (P < 0.01). Test characteristics were calculated for Chlamydia-EIA (Biologische Analysensystem GmbH, Lich, Germany) and pELISA (Medac, Wedel, Germany). pELISA seems to be a good alternative to MIF. It has high specificity and is easier to perform.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Antibodies, Bacterial / blood*
Chlamydia Infections / diagnosis*
Chlamydia trachomatis / immunology*
Female
Fertility
Humans
Immunoassay / methods
Immunoassay / standards
Infertility / microbiology*
Pregnancy
Prevalence
Sensitivity and Specificity
Serologic Tests / methods*
Serologic Tests / standards

Substances

Antibodies, Bacterial