Abstract
A cytosolic acetyl-CoA hydrolase (CACH) cDNA has been isolated from mouse liver cDNA library and sequenced. Recombinant expression of the cDNA in insect cells resulted in overproduction of active acetyl-CoA hydrolyzing enzyme protein. The mouse CACH cDNA encoded a 556-amino-acid sequence that was 93.5% identical to rat CACH, suggesting a conserved role for this enzyme in the mammalian liver. Database searching shows no homology to other known proteins, but reveals homological cDNA sequences showing two single-nucleotide polymorphisms (SNPs) in the CACH coding region. The discovery of mouse CACH cDNA is an important step towards genetic studies on the functional analysis of this enzyme by gene-knockout and transgenic approaches.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyl-CoA Hydrolase / chemistry
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Acetyl-CoA Hydrolase / genetics*
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Acetyl-CoA Hydrolase / metabolism*
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Adenosine Diphosphate / pharmacology
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Adenosine Triphosphate / pharmacology
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Cytosol / enzymology*
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DNA, Complementary / genetics
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Gene Expression
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Lipid Metabolism*
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Liver / enzymology
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Mice
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Molecular Sequence Data
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Spodoptera / cytology
Substances
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DNA, Complementary
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Recombinant Proteins
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Adenosine Diphosphate
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Adenosine Triphosphate
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Acetyl-CoA Hydrolase
Associated data
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GENBANK/AA066584
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GENBANK/AB078618
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GENBANK/AI425375
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GENBANK/AK004905