Abstract
The mechanisms governing centrosome duplication remain poorly understood. We identified a gene called sas-4 that is essential for this process in C. elegans. SAS-4 encodes a predicted coiled-coil protein that localizes to a tiny dot in the center of centrosomes throughout the cell cycle. FRAP experiments with GFP-SAS-4 transgenic embryos reveal that SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication. Additional evidence indicates that SAS-4 is recruited to the daughter centriole or a closely associated structure. These findings identify SAS-4 recruitment as a key step in the centrosome duplication cycle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / metabolism*
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Caenorhabditis elegans Proteins / genetics*
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Caenorhabditis elegans Proteins / metabolism*
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Cell Cycle / genetics*
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Centrioles / genetics
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Centrioles / metabolism
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Centrosome / metabolism*
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Embryo, Nonmammalian / cytology
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Embryo, Nonmammalian / metabolism
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Female
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Fluorescence Resonance Energy Transfer
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Fluorescent Antibody Technique
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Gene Expression Regulation / genetics
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Germ Cells / cytology
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Germ Cells / metabolism
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Green Fluorescent Proteins
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Luminescent Proteins
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Male
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Molecular Sequence Data
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Protein Kinases / deficiency
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Protein Kinases / genetics
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RNA Interference / physiology
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Spermatozoa / cytology
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Spermatozoa / metabolism
Substances
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Caenorhabditis elegans Proteins
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Luminescent Proteins
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SAS-4 protein, C elegans
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Green Fluorescent Proteins
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Protein Kinases
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zyg-1 protein, C elegans