Elevated HGF levels in sera from breast cancer patients detected using a protein microarray ELISA

Ronald L Woodbury; Susan M Varnum; Richard C Zangar

doi:10.1021/pr025506q

Elevated HGF levels in sera from breast cancer patients detected using a protein microarray ELISA

J Proteome Res. 2002 May-Jun;1(3):233-7. doi: 10.1021/pr025506q.

Authors

Ronald L Woodbury¹, Susan M Varnum, Richard C Zangar

Affiliation

¹ Battelle, Pacific Northwest National Laboratory, Molecular Biosciences Department, P7-56, P.O. Box 999, Richland, Washington 99352, USA.

PMID: 12645900
DOI: 10.1021/pr025506q

Abstract

We developed an ELISA in high-density microarray format to detect hepatocyte growth factor (HGF) in human serum. The microassay can detect HGF at sub-pg/mL concentrations in sample volumes of 100 microL or less. The microassay is also quantitative and was used to detect elevated HGF levels in sera from recurrent breast cancer patients. The microarray format provides the potential for high-throughput quantitation of multiple biomarkers in parallel, as demonstrated with a multiplex analysis of five biomarker proteins.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biomarkers, Tumor / blood*
Breast Neoplasms / blood*
Breast Neoplasms / pathology
Enzyme-Linked Immunosorbent Assay / methods*
Female
Hepatocyte Growth Factor / blood*
Humans
Neoplasm Recurrence, Local / blood
Protein Array Analysis / methods*
Reproducibility of Results
Sensitivity and Specificity

Substances

Biomarkers, Tumor
Hepatocyte Growth Factor