Abstract
A protozoa-specific primer (P-SSU-342f) was designed and paired with a eukarya-specific primer to amplify a 1,360-bp fragment of DNA encoding protozoal small subunit (SSU) ribosomal RNA from ruminal fluid of cows fed a mixed forage:grain diet or alfalfa hay. Sequencing of clones showed that P-SSU-342f is specific to ruminal protozoa and, with slight modifications, the primer will be useful for ecological studies of ruminal protozoa.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Animals
-
Base Sequence
-
Cattle
-
DNA Primers
-
DNA, Protozoan / genetics*
-
DNA, Ribosomal / genetics*
-
Eukaryota / classification*
-
Eukaryota / genetics
-
Gene Amplification
-
Molecular Sequence Data
-
Phylogeny
-
Polymerase Chain Reaction / veterinary
-
RNA, Ribosomal, 18S / genetics*
-
Rumen / parasitology*
-
Sequence Alignment / veterinary
Substances
-
DNA Primers
-
DNA, Protozoan
-
DNA, Ribosomal
-
RNA, Ribosomal, 18S