The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA)

J Bacteriol. 2003 Jun;185(11):3476-9. doi: 10.1128/JB.185.11.3476-3479.2003.

Abstract

Mycothiol is the major thiol present in most actinomycetes and is produced from the pseudodisaccharide 1D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins). A transposon mutant of Mycobacterium smegmatis shown to be GlcNAc-Ins and mycothiol deficient was sequenced to identify a putative glycosyltransferase gene designated mshA. The ortholog in Mycobacterium tuberculosis, Rv0486, was used to complement the mutant phenotype.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Cysteine
  • DNA Transposable Elements
  • Disaccharides / biosynthesis*
  • Glycopeptides
  • Glycosyltransferases / genetics*
  • Glycosyltransferases / metabolism*
  • Inositol
  • Molecular Sequence Data
  • Mutation
  • Mycobacterium smegmatis / enzymology*
  • Mycobacterium smegmatis / genetics
  • Mycobacterium tuberculosis / enzymology*
  • Mycobacterium tuberculosis / genetics
  • Pyrazoles
  • Sequence Alignment
  • Sulfhydryl Compounds

Substances

  • Bacterial Proteins
  • DNA Transposable Elements
  • Disaccharides
  • Glycopeptides
  • Pyrazoles
  • Sulfhydryl Compounds
  • mycothiol
  • Inositol
  • Glycosyltransferases
  • Cysteine