Two new peptides to improve post-operative gastric ileus in dog

Peptides. 2003 Apr;24(4):531-4. doi: 10.1016/s0196-9781(03)00113-x.

Abstract

Peptides can influence gastrointestinal motility, and from data obtained earlier in rats, we hypothesized that MTL-RP/Ghrelin, as well as CGRP receptor antagonist 8-37, could improve gastric post-operative ileus in dog. Dogs submitted to laparotomy were perfused with or saline or CGRP 8-37 or MTL-RP/Ghrelin on days 1-4 post-operatively while gastric emptying was estimated by measuring the postprandial increase in plasma acetaminophen ingested with a meal. As expected, in saline-treated animals the gastric emptying function was impaired post-operatively. The total amount of acetaminophen emptied (AUC over 150 min) on post-operative days 1-4 reached respectively 31+/-5%, 65+/-8%, 60+/-8% and 62+/-8% of the normal emptying capacity. CGRP antagonist increased the total emptying of acetaminophen to 52+/-5% on day 1, 95+/-2% on day 2 and 103+/-3% (P<0.05) on day 3. The delayed emptying of acetaminophen seen post-operatively in saline-treated animals could be completely reversed by MTL-RP/Ghrelin (P<0.01) whether it was given at 100 microg/kg on day 2 (102+/-7% of the normal emptying capacity), 4 microg/kg on day 3 (106+/-7%) or 20 microg/kg on day 4 (132+/-8%). As found earlier in rodents, CGRP receptor antagonist 8-37 as well as MTL-RP/Ghrelin are potent prokinetics to improve post-operative gastric ileus in dog.

MeSH terms

  • Acetaminophen / chemistry
  • Acetaminophen / pharmacokinetics
  • Acetaminophen / pharmacology
  • Animals
  • Area Under Curve
  • Calcitonin Gene-Related Peptide / pharmacology
  • Dogs
  • Female
  • Gastric Emptying
  • Ghrelin
  • Ileum / pathology*
  • Ileus / metabolism
  • Kinetics
  • Peptide Fragments / pharmacology
  • Peptide Hormones / pharmacology
  • Peptides / chemistry
  • Peptides / pharmacology*
  • Postoperative Complications*
  • Postprandial Period
  • Stomach / pathology*
  • Time Factors

Substances

  • Ghrelin
  • Peptide Fragments
  • Peptide Hormones
  • Peptides
  • calcitonin gene-related peptide (8-37)
  • Acetaminophen
  • Calcitonin Gene-Related Peptide